進行了一項開放、隨機、人體模型研究，以比較外用抗生素、傷口保護劑和消毒劑對傷口愈合速度和細菌生長的影響，采用改良的方法，使用氫氧化銨誘導的接種金黃色葡萄球菌的皮內水泡。研究中的每位志愿者都有六個水泡傷口（每只前臂三個），每天兩次涂抹三聯抗生素（新霉素、多粘菌素 B、桿菌肽）軟膏或其他四種測試藥物中的一種。對照傷口未經治療。治療后，所有傷口均用封閉敷料覆蓋。評估每個傷口的愈合時間（100% 上皮化）。兩次治療后對傷口進行細菌培養。用三聯抗生素軟膏治療的受污染水泡傷口愈合速度明顯快于用任何消毒劑治療的傷口和未接受治療的傷口只有新霉素-多粘菌素B-桿菌肽組合在兩次應用后（在被金黃色葡萄球菌污染后16至24小時內）才有效消除傷口的細菌污染。用三聯抗生素治療的傷口的整體臨床外觀和愈合率優于研究中除其他抗生素軟膏外的所有治療（和無治療）。

Comparison of topical antibiotic ointments, a wound protectant, and antiseptics for the treatment of human blister wounds contaminated with Staphylococcus aureus

An open, randomized, human-model study was conducted to compare the effects of topical antibiotics, a wound protectant, and antiseptics on the rate of wound healing and bacterial growth using a modification of a method employing ammonium hydroxide-induced intradermal blisters inoculated with Staphylococcus aureus. Each volunteer in the study had six blister wounds (three per forearm) to which a triple antibiotic (neomycin, polymyxin B, bacitracin) ointment or one of four other test agents was applied twice a day. A control wound remained untreated. All wounds were covered with an occlusive dressing after treatment. The time to healing (100 percent epithelialization) was evaluated for each wound. Wounds were cultured for bacterial growth after two treatments. Contaminated blister wounds treated with the triple antibiotic ointment healed significantly faster (mean nine days) than wounds treated with any antiseptic and those receiving no treatment. Only the neomycin-polymyxin B-bacitracin combination effectively eliminated bacterial contamination of the wounds after two applications (within 16 to 24 hours after contamination with Staphylococcus aureus). The overall clinical appearance and healing rates of wounds treated with the triple antibiotic were ranked superior to all treatments (and no treatment) except the other antibiotic ointment in the study.